Antibacterial spectrum of produced reuterin from new isolates of lactobacillus reuteri

Abdulmuttaleb A. Mohammed, Nawfal A. Hussain, Alaa Kareem Niamah

Antibacterial spectrum of produced reuterin from new isolates of lactobacillus reuteri

Číslo: 1/2020/2021
Periodikum: Journal of Microbiology, Biotechnology and Food Sciences
DOI: 10.15414/jmbfs.2020.10.1.134-139

Klíčová slova: Reuterin, Lactobacillus reuteri, antibacterial activity, FT-IR

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Anotace: A total number of 20 samples were collected from different local Iraqi sources. The samples were diluted and inoculated on MRS agar medium. After growth, the isolates were transferred on the modified MRS selective agar medium in order to get isolate of Lactobacillus reuteri which able the reuterin production. Five isolates were obtained from the human infant feces (1-3 months), which were tested by morphological, physio-biochemical assays and molecularly confirmed as L. reuteri by detection of the species gene specific. Electrophoresis of PCR products showed bands at the position 303pb while the sequencing and blasting results of these products were revealed 100% identity of three isolate products to a reference strain L. reuteri ATCC53608 and L. reuteri BPL-36. The fifth residual isolate was limited on physio-biochemical identification as L. reuteri due to failing in sequencing.Zobrazit více »