Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique

Kirill V. Ermakov, Alexander A. Bukhvostov, Alexander S. Vedenkin, Sergey V. Stovbun, Anton S. Dvornikov, Dmitry A. Kuznetsov

Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique

Číslo: 4/2019
Periodikum: Acta Medica
DOI: 10.14712/18059694.2020.8

Klíčová slova: Retinoblastoma, blood plasma, cfDNA, ssDNA, HPLC

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Anotace: A significant population of ultrashort (50–150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL−1), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion – anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.