Anjan Saha, Rakesh Kumar, Mrityunjay Chaubey, Chandan Sengupta, Sauren Das
Efficacy of several qtl and mirna-ssr markers towards health benefit traits in an elite darjeeling tea cultivar
Číslo: 4/2019/2020
Periodikum: Journal of Microbiology, Biotechnology and Food Sciences
Klíčová slova: Antioxidant activity; catechins; microRNA; QTLs; SSRs; tea
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Anotace:
Antioxidant and free radical scavenging ability of tea polyphenols are considered as health benefit traits that mainly contributed by tea
catechins. Therefore understanding of genetic and molecular basis of this secondary metabolite production is of prime interest to
facilitate the crop improvement program in tea plant efficiently. Some QTLs for these quality traits have already been available. In
present study, 10 QTL linked SSRs and 14 newly developed microRNA based SSRs have been characterized in two Darjeeling tea
cultivars having contrast characters and their progeny clone TS569 for validating possible association with catechin contents and
antioxidant activities. Individual catechins and radical scavenging activity have been estimated by high performance liquid
chromatography and UV-Vis spectrophotometry. Some of the polymorphic loci are indicated to be co-varied with such biochemical
physiognomies. Characterization of microRNA based SSRs polymorphism are being the first time report in tea and discussed as ideal
choice for further genotypic studies in tea.
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catechins. Therefore understanding of genetic and molecular basis of this secondary metabolite production is of prime interest to
facilitate the crop improvement program in tea plant efficiently. Some QTLs for these quality traits have already been available. In
present study, 10 QTL linked SSRs and 14 newly developed microRNA based SSRs have been characterized in two Darjeeling tea
cultivars having contrast characters and their progeny clone TS569 for validating possible association with catechin contents and
antioxidant activities. Individual catechins and radical scavenging activity have been estimated by high performance liquid
chromatography and UV-Vis spectrophotometry. Some of the polymorphic loci are indicated to be co-varied with such biochemical
physiognomies. Characterization of microRNA based SSRs polymorphism are being the first time report in tea and discussed as ideal
choice for further genotypic studies in tea.