The effect of fumonisins producing Fusarium verticillioides on the microbiota in pig caecum

Huu Anh Dang, Attila Zsolnai, Melinda Kovács, Brigitta Bóta, Gábor Mihucz, Roland Pósa, Kinga Marosi, Mariam Kachlek, Judit Szabó-Fodor

The effect of fumonisins producing Fusarium verticillioides on the microbiota in pig caecum

Číslo: 1/2019
Periodikum: Acta Veterinaria Brno
DOI: 10.2754/avb201988010065

Klíčová slova: Fumonisin B1, caecal, culturing, qPCR

Pro získání musíte mít účet v Citace PRO.

Přečíst po přihlášení

Anotace: Fumonisin-producing fungal species, Fusarium verticillioides, culture was mixed in the diets of 6 piglets for 9 days (Fumonisin B1 [FB1] intake of 17 mg/kg) to investigate whether there is any potential alteration in the caecal bacterial communities between the experimental (with F. verticillioides) and control groups (without F. verticillioides). Plate count agar culturing technique was applied to measure the amount of aerobic and anaerobic bacteria, Escherichia coli, coliforms, Lactobacillus spp. and Clostridium perfringens. A significant difference was observed between the control and experimental group only in the case of aerobic bacteria on Day 4, 8.60 ± 0.22 compared to 8.06 ± 0.20 (P < 0.05), respectively. Quantitative polymerase chain reaction (qPCR) was performed to estimate the DNA copy number of total bacteria, Bacteroides and Prevotella spp., Clostridium spp., E. coli, Enterobacteriales, Firmicutes and Lactobacillus spp. Significant differences were observed between the control and experimental group regarding total bacteria on Day 2 and Day 6, Firmicutes on Day 2 and E. coli and Enterobacteriales on Day 4. Regarding the entire feeding time, no significant difference between the two groups was found in all species of investigated bacteria by the culturing technique and qPCR after an 8-day exposure. The present research contributes to the understanding of how microbiota responds to the FB1 load.