Supplemental methionine, choline, or taurine affect galectin gene expression in adult holstein cow and neonatal holstein calf neutrophils in vitro

Emmanuel K. Asiamah, Mario Vailati-Riboni, Juan J. Loor, Mulumebet Worku, Hye won Kang

Supplemental methionine, choline, or taurine affect galectin gene expression in adult holstein cow and neonatal holstein calf neutrophils in vitro

Číslo: 1/2020/2021
Periodikum: Journal of Microbiology, Biotechnology and Food Sciences
DOI: 10.15414/jmbfs.2020.10.1.87-92

Klíčová slova: Neutrophils, Galectins, Cows, Calves, Methionine, Choline, Taurine

Pro získání musíte mít účet v Citace PRO.

Přečíst po přihlášení

Anotace: The aim of the study was to evaluate the effect of methyl donor supplementation on mRNA expression of galectin genes in cow and calf neutrophils in vitro. Galectins play a crucial role in homeostasis and disease regulation. Ten adult Holstein cows and five neonatal Holstein calves (3 wk. old) were used for isolation and in vitro culture of neutrophils. Neutrophils isolated from the adult cows were divided into two groups. Neutrophils from Group 1 were incubated with three concentrations of Lysine (Lys) to Met ratios of (3.6:1, 2.9:1, or 2.4:1). Neutrophils from Group 2 were also incubated with three concentrations of Lys to Met ratios and 50 μg /mL of LPS. Isolated neutrophils from calves were incubated with Met, Choline, and Taurine (37 °C, with 5% CO2 or 4 hours). Met did not affect LGALS expression in Group 1 cows.Zobrazit více »