Effect of Different Concentrations ofWhite Tea Extract on theQuality ofAwassi Ram Semen on Chilled Storage

Anotace: The white tea extract (WTE) has compounds that act as antioxidants, which enhance sperm characteristics during storage. Thepresent study was aimed at investigating theeffect ofadding different concentrations ofWTE stored at 4 °C for 24, 48 and 72 h on semen quality. Semen samples were collected from three Awassi rams, and theejaculates were combined. Semen were divided into four equal parts, and it was diluted with Tris; beside theextender, thecontrol group was (A0, without WTE), thesecond group A1 (0.05 mg WTE), thethird group was A2 (0.10 mg WTE), and thefourth group was A3 (0.15 mg WTE). Results revealed thepercentages ofindividual motility, live, and sperm membrane integrity were enhanced significantly (P <0.05) throughout storage in theA2 group compared to other groups. TheA1, A2 and A3 groups decreased significantly (P <0.05) in total sperm abnormality percent at 48 and 72 h. Abnormal head sperm decreased significantly (P <0.05) at 24, 48 and 72 h in theA2 and A3 groups. While theA2 group decreased significantly (P <0.05) in abnormal middle sperm throughout storage. In conclusion, WTE has compounds in it that function as antioxidants. Especially when 0.10 mg ofWTE was added to extenders, there was an improvement in individual motility, live sperm, and sperm membrane integrity, as well as adecrease in total sperm abnormality and types ofabnormal sperm (head, middle, and tail).