Opper sulphate elicitation of optimized suspension culture of andrographis paniculata nees yields unprecedented level of andrographolide

Anilkumar A. Dawande, Sanjay Sahay

Opper sulphate elicitation of optimized suspension culture of andrographis paniculata nees yields unprecedented level of andrographolide

Číslo: 4/2019/2020
Periodikum: Journal of Microbiology, Biotechnology and Food Sciences
DOI: 10.15414/jmbfs.2020.9.4.688-694

Klíčová slova: Andrographis paniculata, Andrographolide, Copper sulphate, Suspension culture, Elicitation

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Anotace: Concerted efforts have been made to push up andrographolide production through optimization of culture conditions, calli induction,

cell growth and elicitation in cell suspension culture of Andrographis paniculata Nees. Optimum callus induction was obtained with
cotyledon and hypocotyls of the plant on Skoog and Hilderbrandt (SH) medium containing 2.0 µg/mL 2,4- dichlorophenoxyacetic acid
and 0.1µg/mL 6-benzyl amino purine (BAP). Half MS medium containing 20 g/L sucrose and 20 h photoperiod showed highest cells
fresh weight (CFW) (17.96 ± 0.06 g/50 mL), growth index (10.95 ± 0.96) and andrographolide yield (4.61±0.688 mg/g DCW). The
addition of copper sulphate (500 µM/L), Methyl jasmonate (25 mg/L), Chitin (500 mg/L) or Fungal mycelium (500 mg/L) in separate
experiments showed significant increase (p=0.05) in bioproduction of andrographolide to the extent of 29.42±0.31 mg/g DCW,
13.13±0.11mg/g DCW, 19.45±0.68 mg/g DCW and 13.629±1.12 mg/g DCW respectively, copper sulphate thus proved to be the most
effective one. The study indicated that a holistic approach involving both culture conditions and elicitation could enhance the overall
production of secondary metabolites (andrographolide) appreciably in this herb and possibly in other medicinal plants.