Anotace:
This study initiates DNA barcoding and phylogenetic investigations of Pseudoleptonema quinquefasciatum Martynov 1935, aiming to enhance species identification, assess genetic diversity, and clarify its evolutionary relationships. By analysing mitochondrial cytochrome c oxidase subunit I (COI) gene sequences from specimens collected in the Northwest Himalaya, we successfully generated the first DNA barcode for P. quinquefasciatum from India. Our findings establish a foundational framework for future taxonomic, ecological, and conservation studies on the genetic structure of P. quinquefasciatum. The generated DNA barcode data can facilitate precise species identification, aiding in resolving cryptic diversity and potential misidentifications in closely related taxa. Furthermore, our phylogenetic analyses shed light on the evolutionary affinities of P. quinquefasciatum within Hydropsychidae, reinforcing the significance of molecular approaches in taxonomy.