Anotace:
Salmonella sp. resistance against various antibiotics is one of the current health problems. An alternative to dealing with Salmonella sp. is using a bacteriophage-based biocontrol. In this study, the isolation of bacteriophages originated from Kencong and Puger areas in Jember Regency East Java, which was prone to food poisoning cases. Eleven bacteriophages were isolated from washing-water samples (φSC1, φSC2, φSC3, φSC4, φSC5), food samples (φSM1, φSM2, φSM3), and fish-waste samples (φSUT, φSP1, φSP2). The result showed that φSC3, φSC4, φSC5, and φSM1 have broad infection capabilities to the genera of Salmonella, Escherichia, and Staphylococcus. All isolates were classified as bacteriophages with DNA nucleic acids. The TEM morphological observations indicated that φSC4 isolates belong to the Podoviridae while φSP1 isolates belong to the Myoviridae. These results suggest that the phages could potentially be used for biocontrol purposes. However, further characterization, viability measurement and activity are needed before their use in food applications against foodborne pathogens.