Terezie Zavadilova, Zbysek Sladek, Lucie Kratochvilova, Petr Slama, Dusan Rysanek
The effect of muramyl dipeptide and lipopolysaccharide on expression of cd14 and cd44 by bovine mammary gland neutrophils in vitro
Číslo: Special issue/2019/2020
Periodikum: Journal of Microbiology, Biotechnology and Food Sciences
DOI: 10.15414/jmbfs.2019.9.special.467-472
Klíčová slova: heifer, mammary gland, inflammation, apoptosis
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undertaken in vitro investigates whether bacterial components affect neutrophils’ CD14 and CD44 expression and lifespan. Neutrophils,
obtained through lavage of mammary glands, were incubated with three different concentrations of muramyl dipeptide (MDP) and
lipopolysaccharide (LPS). Percentages of apoptotic neutrophils, and of CD14+ and CD44+ neutrophils, were detected after 30, 60, 120
and 300 minutes of incubation. A high concentration of MDP produced a significantly higher percentage of CD14+ after 30 and 120
minutes, compared with LPS and the control, for which percentages remained constant. CD44+ increased at low concentrations of MDP
and LPS after 120 minutes, and at 60 minutes for intermediate concentrations. High concentrations resulted in significant increase, this
after only 30 minutes for MDP. Analysis of variance indicated incubation time followed by bacterial component, as most important in
expression of CD14 and CD44.