Possibilities of lectin helix pomatia agglutinin (hpa) use in rabbits insemination

Martin Fik

Possibilities of lectin helix pomatia agglutinin (hpa) use in rabbits insemination

Číslo: 3/2018/2019
Periodikum: Journal of Microbiology, Biotechnology and Food Sciences
DOI: 10.15414/jmbfs.2018-

Klíčová slova: Spermatozoa, rabbit, insemination, Helix pomatia agglutinin, lectin

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Anotace: The aim of this work was to analyse the effect of incubation HPA (lectin Helix pomatia agglutinin) with ID (insemination dose) on reproduction parameters (conception ratio, number of live and dead born of pups and birth weight of pups) of rabbits. The experiments were carried out under conditions of 2 rabbit breeds (production breed P and experimental breed E). Fresh ID, in sperm concentrations 25 mil. / 0.5 ml ID, were divided into control ID (without HPA) and experimental ID (with HPA). HPA was added to the experimental ID in concentrations 1µg HPA / 1x106 spermatozoa (30 - 60 minutes of incubation before AI – artificial insemination). Conception ratios obtained from 2 different breeds (P, E) showed minor improvement ( + 2.67 % P; + 7.36 % E) between the monitored groups of females, however without statistically significant differences (P χ2 = 0.03; E χ2 = 0.11). Number of live born pups in litter obtained from breeds P and E showed minor improvement (+ 0.37 pcs P (p>0.05); + 0.95 pcs E (p≤0.05)) compared to the control groups. The weight of live pups among the monitored groups was comparable. It can be concluded from the present study that incubation of ID with lectin HPA had been positively affected by the observed reproductive parameters. The statistically significant difference was found only in the number of live-born pups in the litter. The price of lectin is too high for breeding in common breeding practice.